Journal: Cancers
Article Title: Automated Capture and Analysis of Circulating Tumor Cells in Pediatric, Adolescent and Young Adult Patients with Central Nervous System Tumors
doi: 10.3390/cancers15153853
Figure Lengend Snippet: Circulating Tumor Cell (CTC) Isolation Workflow. Freshly collected (<24 h) patient peripheral blood is subjected to gradient centrifugation (1) to isolate peripheral blood mononuclear cells (PBMCs) (2). PBMCs are separated and collected into a 200 µL suspension (3) which is loaded onto the Abnova CytoQuest CTC isolation platform (4). After automated processing, CTCs are bound to the cell surface vimentin (CSV)-coated CTC chip (5). Cells captured on the chip are then stained (CSV, CD45, DAPI) and imaged.
Article Snippet: All antibodies used for this process are part of the CytoQuest™ Universal Circulating Tumor Cell CSV CD45 Antibody Kit (KA4818 Abnova).
Techniques: Isolation, Gradient Centrifugation, Suspension, Staining